Journal: Heliyon

Article Title: Serum spectrin breakdown product and neurofilament heavy in predicting outcome after cardiac arrest: A diagnostic accuracy study

doi: 10.1016/j.heliyon.2023.e22582

Figure Lengend Snippet: Contingency table and performance of biomarkers in predicting poor outcome 6 months after cardiac arrest.

Article Snippet: We used commercially available enzyme-linked immunosorbent assay kit for the SBDP145 (MBS7606325, MyBioSource, San Diego, CA, USA) and Nf-H (NBP2-78763, Novus Biologicals, Englewood, CO, USA) analyses.

Techniques:

Journal: Arthritis and rheumatism

Article Title: Neuroplasticity of sensory and sympathetic nerve fibers in a mouse model of a painful arthritic joint.

doi: 10.1002/art.34385

Figure Lengend Snippet: Figure 5. Confocal images showing that CFA-induced inflammation of the joint induces sprouting of NF200, CGRP, and GAP-43 nerve fibers in the synovium and this sprouting is substantially attenuated by anti-NGF therapy. Representative confocal images of NF200- and CGRP-labeled sensory nerve fibers and GAP-43–labeled sprouting nerve fibers (yellow/orange) and DAPI-labeled nuclei (blue) in 20-m–thick knee joint sections from sham (saline)–injected mice (A, D, and G), CFA-injected mice treated with vehicle (B, E, and H), and CFA-injected mice treated with anti-NGF (C, F, and I) are shown. In sham-injected mice, a low-level, regular pattern of innervation by NF200, CGRP, and GAP-43 fibers was observed in the synovial space of the knee joint. Twenty-eight days after the initial injection of CFA, a significant number of NF200, CGRP, and GAP-43 nerve fibers had sprouted and exhibited a disorganized appearance as compared to observations in joints treated with saline only. NF200, CGRP, and GAP-43 sprouted nerve fibers were localized in the synovium and were not observed in the meniscus. Administration of anti-NGF significantly reduced the density of NF200, CGRP, and GAP-43 nerve fibers in the synovium of the joints with CFA-induced arthritis. See Figure 4 for definitions.

Article Snippet: Frozen bone/knee joint sections were incubated with an antibody against calcitonin gene– related peptide (CGRP) (polyclonal rabbit anti-rat CGRP; 1:10,000) (catalog no. C8198; Sigma) to label unmyelinated and thinly myelinated primary afferent sensory nerve fibers, and an antibody against neurofilament 200 kd (NF200) (chicken anti-NF200; 1:5,000) (catalog no. CH22104; Neuromics) to label myelinated primary afferent sensory nerve fibers.

Techniques: Labeling, Saline, Injection

Journal: Arthritis and rheumatism

Article Title: Neuroplasticity of sensory and sympathetic nerve fibers in a mouse model of a painful arthritic joint.

doi: 10.1002/art.34385

Figure Lengend Snippet: Figure 4. Anti–nerve growth factor (anti-NGF) reduces Freund’s complete adjuvant (CFA)–induced nerve sprouting of sensory and sympathetic nerve fibers but does not alter the aberrant neovascularization and macrophage infiltration in the arthritic joint. Twenty-eight days after initial injection of CFA, there were significant increases in the density of sensory nerve fibers as demonstrated by positivity for calcitonin gene–related peptide (CGRP) (A) and neurofilament 200 kd (NF200) (B), sympathetic nerve fibers as demonstrated by positivity for tyrosine hydroxylase (TH) (C), and nerve fibers undergoing sprouting as demonstrated by positivity for growth-associated protein 43 (GAP-43) (D), as well as increases in vascularization as demonstrated by CD31 vessels (E) and a significant influx of macrophages (CD68) (F), in the synovium of the arthritic joint. Administration of anti-NGF significantly reduced the density of CGRP and NF200 sensory, TH sympathetic, and GAP-43 sprouting nerve fibers in the synovium of the joints with CFA-induced arthritis. In contrast, preventive sequestration of NGF did not reduce the number or alter the density of the CFA-induced aberrant CD31 neovascularization or the number of CD68 macrophages in the synovium of the arthritic joint. Values are the mean SEM from 6 mice.

Article Snippet: Frozen bone/knee joint sections were incubated with an antibody against calcitonin gene– related peptide (CGRP) (polyclonal rabbit anti-rat CGRP; 1:10,000) (catalog no. C8198; Sigma) to label unmyelinated and thinly myelinated primary afferent sensory nerve fibers, and an antibody against neurofilament 200 kd (NF200) (chicken anti-NF200; 1:5,000) (catalog no. CH22104; Neuromics) to label myelinated primary afferent sensory nerve fibers.

Techniques: Adjuvant, Injection